Hotspot

matK-trnK-UUU

LSC · 3318-4143 · 826 bp

Back to Curcuma

Pi 0.00525

MarkerSeek score 58.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 26
pi: 0.00525
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGAAACTGTTGTTGCCGAGA	TGAAGGGTTGGGTTGCTAAC	45.0	50.0	57.7	58.0	919-932 bp	1.0	82.6
matK-trnK-UUU_p2	AGAAACTGTTGTTGCCGAGA	GTTTTGAAGGGTTGGGTTGC	45.0	50.0	57.7	58.1	923-936 bp	1.0	82.2
matK-trnK-UUU_p3	AGAAACTGTTGTTGCCGAGA	TTTTGAAGGGTTGGGTTGCT	45.0	45.0	57.7	58.1	922-935 bp	1.0	81.6
matK-trnK-UUU_p4	AGAAACTGTTGTTGCCGAGA	AACGGTAGAGTACTCGGCTT	45.0	50.0	57.7	58.2	897-910 bp	1.0	81.4
matK-trnK-UUU_p5	AGAAACTGTTGTTGCCGAGA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.7	58.2	896-909 bp	1.0	81.4