Hotspot

ycf1

Genome · 31602-38750 · 7149 bp

Back to Cupressus

Pi 0.0162

MarkerSeek score 42.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 7149
variable_sites: 212
pi: 0.0162
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTAGACGTAGGCCTAGTCCG	TGGATACCGAGACCAGAGTC	55.0	55.0	58.0	57.9	803-860 bp	1.0	89.1
ycf1_p2	TTAGACGTAGGCCTAGTCCG	CGAATGGGAGCAAGATTGGA	55.0	50.0	58.0	58.0	480-534 bp	0.75	74.0
ycf1_p3	TGAAAAACGTACGCTTGGGA	CGAATGGGAGCAAGATTGGA	45.0	50.0	58.0	58.0	1002-1056 bp	0.75	73.7
ycf1_p4	TCTCCAGGATGCTTGCAATC	CGAATGGGAGCAAGATTGGA	50.0	50.0	58.0	58.0	1146-1200 bp	0.75	73.6
ycf1_p5	CTCTCCAGGATGCTTGCAAT	CGAATGGGAGCAAGATTGGA	50.0	50.0	57.9	58.0	1147-1201 bp	0.75	73.6