Hotspot

rpoA

Genome · 12530-13570 · 1041 bp

Back to Cupressus

Pi 0.0328

MarkerSeek score 71.70

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: Genome
length_bp: 1041
variable_sites: 63
pi: 0.0328
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TATGCTACGTGGCAATGGTC	CGTCGTCCAGTAGCTACAAC	50.0	55.0	58.1	58.1	1564-1915 bp	1.0	85.4
rpoA_p2	TATGCTACGTGGCAATGGTC	AATGGCTTCAGTACCTGCAG	50.0	50.0	58.1	57.9	1637-1988 bp	1.0	85.0
rpoA_p3	CCATTTGCTGCTCAGATTGC	CGTCGTCCAGTAGCTACAAC	50.0	55.0	58.1	58.1	1641-1992 bp	1.0	84.9
rpoA_p4	CCAGCACTGCGAGCTATTTA	CGTCGTCCAGTAGCTACAAC	50.0	55.0	58.1	58.1	1533-1884 bp	0.75	75.0
rpoA_p5	CCAGCACTGCGAGCTATTTA	AATGGCTTCAGTACCTGCAG	50.0	50.0	58.1	57.9	1606-1957 bp	0.75	74.6