Hotspot

psbD-trnT-GGU

Genome · 88618-89482 · 865 bp

Back to Cupressus

Pi 0.00637

MarkerSeek score 61.70

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 865
variable_sites: 11
pi: 0.00637
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	AATAAACCGGACCAACCCAC	GGCGTAAGTCATCGGTTCAA	50.0	50.0	58.1	58.3	1006-1013 bp	1.0	78.4
psbD-trnT-GGU_p2	CAATAAACCGGACCAACCCA	GGCGTAAGTCATCGGTTCAA	50.0	50.0	58.1	58.3	1007-1014 bp	1.0	78.4
psbD-trnT-GGU_p3	AATAAACCGGACCAACCCAC	TAAGTCATCGGTTCAAGCCC	50.0	50.0	58.1	57.6	1002-1009 bp	1.0	77.5
psbD-trnT-GGU_p4	CAATAAACCGGACCAACCCA	TAAGTCATCGGTTCAAGCCC	50.0	50.0	58.1	57.6	1003-1010 bp	1.0	77.5
psbD-trnT-GGU_p5	ACAAAACGGTCTCTGCGTAG	GGCGTAAGTCATCGGTTCAA	50.0	50.0	58.2	58.3	982-989 bp	1.0	77.4