Hotspot

rrn16-trnI-GAU

Genome · 55060-55925 · 866 bp

Back to Cupressus

Pi 0.0143

MarkerSeek score 72.70

Primer available yes

Label rrn16-trnI-GAU

Feature Metadata

label_name: rrn16-trnI-GAU
feature_type: igs
region: Genome
length_bp: 866
variable_sites: 22
pi: 0.0143
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnI-GAU_p1	AGTGACTGGAGTGAAGTCGT	GCGTTGATGAGCACATTGAA	50.0	45.0	58.3	57.4	959-1038 bp	1.0	78.4
rrn16-trnI-GAU_p2	AAGTCGTAACAAGGTAGCCG	GCGTTGATGAGCACATTGAA	50.0	45.0	57.6	57.4	946-1025 bp	1.0	78.0
rrn16-trnI-GAU_p3	GGCTAGTGACTGGAGTGAAG	GCGTTGATGAGCACATTGAA	55.0	45.0	57.3	57.4	963-1042 bp	1.0	75.5
rrn16-trnI-GAU_p4	TGCCATGCTCGAAGTCATTA	GCGTTGATGAGCACATTGAA	45.0	45.0	57.3	57.4	1016-1095 bp	1.0	75.2
rrn16-trnI-GAU_p5	AGTCGTAACAAGGTAGCCGT	GCGTTGATGAGCACATTGAA	50.0	45.0	58.7	57.4	945-1024 bp	1.0	74.8