Hotspot

rps12-clpP

Genome · 28081-29276 · 1196 bp

Back to Cupressus

Pi 0.0183
MarkerSeek score 59.50
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
Genome
length_bp
1196
variable_sites
42
pi
0.0183
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 GCATACTCCCTTCCGTTGAG CCCGTGTAGAAGGAAAGCTC 55.0 55.0 58.1 58.0 1561-1673 bp 1.0 83.0
rps12-clpP_p2 GCATACTCCCTTCCGTTGAG CCGTGTAGAAGGAAAGCTCC 55.0 55.0 58.1 58.0 1560-1672 bp 1.0 83.0
rps12-clpP_p3 GCATACTCCCTTCCGTTGAG CGTGTAGAAGGAAAGCTCCC 55.0 55.0 58.1 58.0 1559-1671 bp 1.0 83.0
rps12-clpP_p4 TTTCTTATCGGCTGTCTCGC CCGTGTAGAAGGAAAGCTCC 50.0 55.0 58.1 58.0 1508-1620 bp 1.0 82.9
rps12-clpP_p5 TTTCTTATCGGCTGTCTCGC CGTGTAGAAGGAAAGCTCCC 50.0 55.0 58.1 58.0 1507-1619 bp 1.0 82.9