Hotspot

accD-rpl23

Genome · 4024-5389 · 1366 bp

Back to Cupressus

Pi 0.0256

MarkerSeek score 80.10

Primer available yes

Label accD-rpl23

Feature Metadata

label_name: accD-rpl23
feature_type: igs
region: Genome
length_bp: 1366
variable_sites: 60
pi: 0.0256
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-rpl23_p1	CATGGTTCCACGAGCTCTTT	TTGATCCCGAATCGACATTT	50.0	40.0	58.2	55.1	1471-1718 bp	1.0	62.7
accD-rpl23_p2	CATGGTTCCACGAGCTCTTT	TTTGATCCCGAATCGACATT	50.0	40.0	58.2	55.1	1472-1719 bp	1.0	62.7
accD-rpl23_p3	CATGGTTCCACGAGCTCTTT	TTTGATCCCGAATCGACATTT	50.0	38.1	58.2	56.0	1472-1719 bp	1.0	61.2
accD-rpl23_p4	CATGGTTCCACGAGCTCTTT	ATTTGATCCCGAATCGACAT	50.0	40.0	58.2	54.9	1473-1720 bp	1.0	60.8
accD-rpl23_p5	ATGGTTCCACGAGCTCTTTT	TTGATCCCGAATCGACATTT	45.0	40.0	57.4	55.1	1470-1717 bp	1.0	59.6