Hotspot

trnP-UGG-psaJ

LSC · 70633-71075 · 443 bp

Back to Cuphea

Pi 0.0754
MarkerSeek score 83.80
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
443
variable_sites
80
pi
0.0754
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 521-602 bp 1.0 90.9
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 520-601 bp 1.0 90.9
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 532-613 bp 1.0 90.8
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 531-612 bp 1.0 90.8
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 534-615 bp 1.0 90.8