Hotspot

rbcL-accD

LSC · 60220-61014 · 795 bp

Back to Cuphea

Pi 0.0513
MarkerSeek score 84.50
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
795
variable_sites
101
pi
0.0513
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 TTGAATGCTACTGCGGGTAC TCAAGCCGCTTATCGTTTGA 50.0 45.0 58.0 57.9 1595 bp 0.142857 44.9
rbcL-accD_p2 TCATGGTATGCACTTCCGTG TCAAGCCGCTTATCGTTTGA 50.0 45.0 58.0 57.9 1395 bp 0.142857 44.8
rbcL-accD_p3 GGGCTGTATTTGCGAGAGAA TCAAGCCGCTTATCGTTTGA 50.0 45.0 58.0 57.9 1555 bp 0.142857 44.8
rbcL-accD_p4 AAGGGCTGTATTTGCGAGAG TCAAGCCGCTTATCGTTTGA 50.0 45.0 58.0 57.9 1557 bp 0.142857 44.8
rbcL-accD_p5 GCTTGTGAAGTGTGGAAGGA TCAAGCCGCTTATCGTTTGA 50.0 45.0 58.0 57.9 941 bp 0.142857 44.8