Pi
0.0594
MarkerSeek score
84.00
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | CGAAGCCATACCCAATCGAA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1448-1545 bp | 1.0 | 91.5 |
| trnT-UGU-trnL-UAA_p2 | CGAAGCCATACCCAATCGAA | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 1456-1553 bp | 1.0 | 91.0 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 754-825 bp | 1.0 | 90.9 |
| trnT-UGU-trnL-UAA_p4 | CGAAGCCATACCCAATCGAA | AAAATAGAGATTCGCGCCGT | 50.0 | 45.0 | 58.1 | 58.1 | 1796-1901 bp | 0.714286 | 77.8 |
| trnT-UGU-trnL-UAA_p5 | CGTTGACCCTTTTGCCTTTC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.9 | 58.0 | 1382-1476 bp | 0.571429 | 70.3 |