Hotspot

trnK-UUU-rps16

LSC · 4426-5307 · 882 bp

Back to Cuphea

Pi 0.075
MarkerSeek score 82.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
882
variable_sites
148
pi
0.075
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.93
markerseek_score
82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CTTCCCCTTACCGGTCACTA GCAATTGATGTGCGATCTCG 55.0 50.0 58.1 58.0 1411-1504 bp 0.857143 85.0
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GCAATTGATGTGCGATCTCG 50.0 50.0 58.2 58.0 1042-1138 bp 0.857143 84.8
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GCAATTGATGTGCGATCTCG 50.0 50.0 58.2 58.0 1041-1137 bp 0.857143 84.8
trnK-UUU-rps16_p4 GAAAACCCTTCCCCTTACCG GCAATTGATGTGCGATCTCG 55.0 50.0 58.2 58.0 1418-1511 bp 0.857143 84.8
trnK-UUU-rps16_p5 CGGAAAACCCTTCCCCTTAC GCAATTGATGTGCGATCTCG 55.0 50.0 58.2 58.0 1420-1513 bp 0.428571 64.6