Hotspot

ycf2

Genome · 61529-68563 · 7035 bp

Back to Cunninghamia

Pi 0

MarkerSeek score 55.20

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 7035
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GAATAAGTTGTATTAACTATGTACTTC	AGCATCTTGTCGCCTTTTCA	25.9	45.0	52.7	58.1	1161 bp	1.0	40.0
ycf2_p2	GAATAAGTTGTATTAACTATGTACTTC	TGAAAAGCATCTTGTCGCCT	25.9	45.0	52.7	58.1	1166 bp	1.0	40.0
ycf2_p3	GAATAAGTTGTATTAACTATGTACTTC	GCATCTTGTCGCCTTTTCAA	25.9	45.0	52.7	57.3	1160 bp	1.0	40.0
ycf2_p4	GAATAAGTTGTATTAACTATGTACTTC	TTGAAAAGCATCTTGTCGCC	25.9	45.0	52.7	57.3	1167 bp	1.0	40.0
ycf2_p5	GAATAAGTTGTATTAACTATGTACTTC	AAGCATCTTGTCGCCTTTTC	25.9	45.0	52.7	57.0	1162 bp	1.0	40.0