Hotspot

rps16

Genome · 4738-5798 · 1061 bp

Back to Cunninghamia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: Genome
length_bp: 1061
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GGACCTAGACGTAGACGCAT	TCCATTTTGTATCAGATCAAAGA	55.0	30.4	58.7	53.7	1115 bp	1.0	40.0
rps16_p2	GGACCTAGACGTAGACGCAT	TCCATTTTGTATCAGATCAAAGATCT	55.0	30.8	58.7	56.7	1115 bp	1.0	40.0
rps16_p3	TTGGACCTAGACGTAGACGC	TCCATTTTGTATCAGATCAAAGA	55.0	30.4	58.9	53.7	1117 bp	1.0	40.0
rps16_p4	TTGGACCTAGACGTAGACGC	TCCATTTTGTATCAGATCAAAGATCT	55.0	30.8	58.9	56.7	1117 bp	1.0	40.0
rps16_p5	GGACCTAGACGTAGACGCAT	TCCATTTTGTATCAGATCAAAGATC	55.0	32.0	58.7	55.5	1115 bp	1.0	40.0