Hotspot

rps12-ycf1

Genome · 93997-95756 · 1760 bp

Back to Cunninghamia

Pi 0.000635

MarkerSeek score 61.80

Primer available yes

Label rps12-ycf1

Feature Metadata

label_name: rps12-ycf1
feature_type: igs
region: Genome
length_bp: 1760
variable_sites: 1
pi: 0.000635
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-ycf1_p1	CCTTTACGCTGAGGACATCC	ATCTGAGCTGGACCAATTGG	55.0	50.0	58.1	57.6	1776-1962 bp	1.0	80.1
rps12-ycf1_p2	ATTGGCTGTCTCGCATTTCT	ATCTGAGCTGGACCAATTGG	45.0	50.0	57.9	57.6	1725-1911 bp	1.0	79.6
rps12-ycf1_p3	GCACATACACCCTAGCACAT	ATCTGAGCTGGACCAATTGG	50.0	50.0	57.7	57.6	1799-1985 bp	1.0	77.9
rps12-ycf1_p4	ACTCCTTTACGCTGAGGACA	ATCTGAGCTGGACCAATTGG	50.0	50.0	58.4	57.6	1779-1965 bp	1.0	77.6
rps12-ycf1_p5	CCTTTACGCTGAGGACATCC	GAGCTGGACCAATTGGCAAT	55.0	50.0	58.1	58.8	1772-1958 bp	1.0	77.1