Hotspot

trnI-trnV

Genome · 52621-55578 · 2958 bp

Back to Cunninghamia

Pi 0.000678

MarkerSeek score 56.30

Primer available yes

Label trnI-trnV

Feature Metadata

label_name: trnI-trnV
feature_type: igs
region: Genome
length_bp: 2958
variable_sites: 2
pi: 0.000678
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-trnV_p1	TACCACTGAGCTAATAGCCC	AGTTCAAACCTGATTATCCCT	50.0	38.1	56.1	54.2	2994-3000 bp	1.0	40.2
trnI-trnV_p2	ACCACTGAGCTAATAGCCC	CAGTTCAAACCTGATTATCCCT	52.6	40.9	56.2	55.8	2994-3000 bp	1.0	40.2
trnI-trnV_p3	CTACCACTGAGCTAATAGCCC	GTTCAAACCTGATTATCCCT	52.4	40.0	57.3	52.5	2994-3000 bp	1.0	40.2
trnI-trnV_p4	TACCACTGAGCTAATAGCCC	AGTTCAAACCTGATTATCCC	50.0	40.0	56.1	52.5	2994-3000 bp	1.0	40.2
trnI-trnV_p5	TACCACTGAGCTAATAGCCC	GTTCAAACCTGATTATCCCT	50.0	40.0	56.1	52.5	2993-2999 bp	1.0	40.2