Hotspot

ycf3-psaA

Genome · 11801-12409 · 609 bp

Back to Cunninghamia

Pi 0
MarkerSeek score 58.90
Primer available yes
Label ycf3-psaA

Feature Metadata

label_name
ycf3-psaA
feature_type
igs
region
Genome
length_bp
609
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-psaA_p1 TGGTTTGATCAAGCTGCTGA AGAAATGACCGGGTTTAGCC 45.0 50.0 57.7 57.9 813-814 bp 1.0 76.9
ycf3-psaA_p2 TGGTTTGATCAAGCTGCTGA GGATCCCTCTCCACTACGAT 45.0 55.0 57.7 57.7 769-770 bp 1.0 75.5
ycf3-psaA_p3 TGGAAACAAGCTATAGCGCT AGAAATGACCGGGTTTAGCC 45.0 50.0 57.3 57.9 789-790 bp 1.0 73.6
ycf3-psaA_p4 TGGAAACAAGCTATAGCGCT GGATCCCTCTCCACTACGAT 45.0 55.0 57.3 57.7 745-746 bp 1.0 72.2
ycf3-psaA_p5 GGTTTGATCAAGCTGCTGAG AGAAATGACCGGGTTTAGCC 50.0 50.0 57.1 57.9 812-813 bp 1.0 71.8