Hotspot

matK-trnK

Genome · 3289-3985 · 697 bp

Back to Cunninghamia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK

Feature Metadata

label_name: matK-trnK
feature_type: igs
region: Genome
length_bp: 697
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK_p1	TGCGTAAAGATCTTCCCGAA	ACTGCGTATTCCACTTCGAC	45.0	50.0	57.0	58.0	861 bp	1.0	71.6
matK-trnK_p2	TGCGTAAAGATCTTCCCGAA	GCGTATTCCACTTCGACTGT	45.0	50.0	57.0	58.0	858 bp	1.0	71.6
matK-trnK_p3	TTGCGTAAAGATCTTCCCGA	ACTGCGTATTCCACTTCGAC	45.0	50.0	57.0	58.0	862 bp	1.0	71.6
matK-trnK_p4	TTGCGTAAAGATCTTCCCGA	GCGTATTCCACTTCGACTGT	45.0	50.0	57.0	58.0	859 bp	1.0	71.6
matK-trnK_p5	TGCGTAAAGATCTTCCCGAA	TGCGTATTCCACTTCGACTG	45.0	50.0	57.0	58.0	859 bp	1.0	71.6