Hotspot

ycf1

SSC · 127638-133166 · 5529 bp

Back to Cucurbita

Pi 0.00752

MarkerSeek score 50.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5529
variable_sites: 98
pi: 0.00752
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTCGGGGAGAACCAAAAGG	CCTTACCGCAGACGAATCAA	50.0	50.0	58.0	58.0	1283-1304 bp	1.0	84.6
ycf1_p2	TTTCGGGGAGAACCAAAAGG	AGCTCGAGTAGGGGATGTAC	50.0	55.0	58.0	58.0	1383-1404 bp	1.0	84.3
ycf1_p3	TTTCGGGGAGAACCAAAAGG	GCTCGAGTAGGGGATGTACT	50.0	55.0	58.0	58.0	1382-1403 bp	1.0	84.3
ycf1_p4	TTTCGGGGAGAACCAAAAGG	CAGCTCGAGTAGGGGATGTA	50.0	55.0	58.0	58.0	1384-1405 bp	1.0	84.2
ycf1_p5	TTTCGGGGAGAACCAAAAGG	TGCCAAAGTGACGGAAAAGA	50.0	45.0	58.0	58.0	1598-1619 bp	1.0	83.5