Hotspot

trnL-trnF

LSC · 50914-52194 · 1281 bp

Back to Cucurbita

Pi 0.0371

MarkerSeek score 81.60

Primer available yes

Label trnL-trnF

Feature Metadata

label_name: trnL-trnF
feature_type: igs
region: LSC
length_bp: 1281
variable_sites: 72
pi: 0.0371
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-trnF_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	863-1371 bp	1.0	84.6
trnL-trnF_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	860-1368 bp	1.0	81.4
trnL-trnF_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	852-1360 bp	1.0	80.8
trnL-trnF_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	50.0	55.0	57.5	58.6	825-1333 bp	1.0	80.5
trnL-trnF_p5	AATCGTGAGGGTTCAAGTCC	TCATGATGTGCCAAGAACCA	50.0	45.0	57.5	57.4	885-1393 bp	1.0	80.3