Hotspot

psbZ-trnG

LSC · 38620-39167 · 548 bp

Back to Cucurbita

Pi 0.00295

MarkerSeek score 57.70

Primer available yes

Label psbZ-trnG

Feature Metadata

label_name: psbZ-trnG
feature_type: igs
region: LSC
length_bp: 548
variable_sites: 4
pi: 0.00295
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG_p1	CGTTGCATTTGCTTCTCCTG	AATCGAACCCGCATCTTCTC	50.0	50.0	58.0	58.1	717-724 bp	1.0	80.7
psbZ-trnG_p2	CGTTGCATTTGCTTCTCCTG	GAATCGAACCCGCATCTTCT	50.0	50.0	58.0	58.1	718-725 bp	1.0	80.6
psbZ-trnG_p3	TTGGTCGGCGAACAAAAATG	AATCGAACCCGCATCTTCTC	45.0	50.0	57.9	58.1	693-700 bp	1.0	79.9
psbZ-trnG_p4	TTGGTCGGCGAACAAAAATG	GAATCGAACCCGCATCTTCT	45.0	50.0	57.9	58.1	694-701 bp	1.0	79.9
psbZ-trnG_p5	GGTCGGCGAACAAAAATGTT	AATCGAACCCGCATCTTCTC	45.0	50.0	57.9	58.1	691-698 bp	1.0	79.9