Hotspot

trnN-GUU

SSC · 131299-131370 · 72 bp

Back to Cucumis

Pi 0.0283

MarkerSeek score 50.30

Primer available yes

Label trnN-GUU

Feature Metadata

label_name: trnN-GUU
feature_type: tRNA
region: SSC
length_bp: 72
variable_sites: 4
pi: 0.0283
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU_p1	CCGTTGTTCCTCAGTAGCTC	GGGAACGGGTTACCTACTCT	55.0	55.0	58.0	58.1	155 bp	1.0	80.9
trnN-GUU_p2	TGGGTTGAGAACGGGAATTG	GGGAACGGGTTACCTACTCT	50.0	55.0	58.1	58.1	196 bp	1.0	79.7
trnN-GUU_p3	TTGGTCATGGGTTGAGAACG	GGGAACGGGTTACCTACTCT	50.0	55.0	58.1	58.1	203 bp	1.0	79.6
trnN-GUU_p4	CATGGGTTGAGAACGGGAAT	GGGAACGGGTTACCTACTCT	50.0	55.0	57.9	58.1	198 bp	1.0	79.4
trnN-GUU_p5	CCGTTGTTCCTCAGTAGCTC	AACAAAGACACAGGGAACGG	55.0	50.0	58.0	58.3	167 bp	1.0	79.3