Hotspot

clpP

LSC · 72361-74089 · 1729 bp

Back to Cucumis

Pi 0.0184

MarkerSeek score 44.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1729
variable_sites: 94
pi: 0.0184
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATGGTCTAAACGAGTCGCAC	GAACAGAAAGCAAACGACCG	50.0	50.0	58.0	58.0	2560-2822 bp	1.0	83.8
clpP_p2	GGTCTAAACGAGTCGCACAT	GAACAGAAAGCAAACGACCG	50.0	50.0	58.0	58.0	2558-2820 bp	1.0	83.8
clpP_p3	CATGGTCTAAACGAGTCGCA	GAACAGAAAGCAAACGACCG	50.0	50.0	58.0	58.0	2561-2823 bp	1.0	83.8
clpP_p4	ACTTTCTTTTGTCCCGGTCC	GAACAGAAAGCAAACGACCG	50.0	50.0	58.0	58.0	2580-2842 bp	1.0	83.6
clpP_p5	CTTTCTTTTGTCCCGGTCCA	GAACAGAAAGCAAACGACCG	50.0	50.0	58.0	58.0	2579-2841 bp	1.0	83.6