Hotspot

trnL-UAG-ccsA

SSC · 115603-115706 · 104 bp

Back to Cucumis

Pi 0.0283

MarkerSeek score 44.70

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 104
variable_sites: 9
pi: 0.0283
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	TGCCCTTTTCTGACGAAACA	50.0	45.0	58.0	58.0	308-311 bp	1.0	82.9
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	TGCCCTTTTCTGACGAAACA	50.0	45.0	58.0	58.0	307-310 bp	1.0	82.9
trnL-UAG-ccsA_p3	GAGAAAGCCGCTATGGTGAA	TGCCCTTTTCTGACGAAACA	50.0	45.0	58.0	58.0	323-326 bp	1.0	82.6
trnL-UAG-ccsA_p4	ATGATTTCGAGAAAGCCGCT	TGCCCTTTTCTGACGAAACA	45.0	45.0	58.0	58.0	331-334 bp	1.0	82.5
trnL-UAG-ccsA_p5	GTGAAATCGGTAGACACGCT	AAATAGTACTTGGCGCGGTT	50.0	45.0	58.0	57.9	503-506 bp	1.0	82.0