Hotspot

trnL-CAA-ndhB

IRb · 95621-96198 · 578 bp

Back to Cucumis

Pi 0.00187

MarkerSeek score 45.50

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 578
variable_sites: 3
pi: 0.00187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	CCAGGAATATCAATGAACCCGA	50.0	45.5	58.0	57.9	545-664 bp	1.0	67.6
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	CCAGGAATATCAATGAACCCGA	50.0	45.5	58.0	57.9	543-662 bp	1.0	67.6
trnL-CAA-ndhB_p3	TTCAACGGGCATTCTCAACA	CCAGGAATATCAATGAACCCGA	45.0	45.5	58.0	57.9	617-736 bp	1.0	67.5
trnL-CAA-ndhB_p4	TACCATTTCACCACCAAGGC	CCAGGAATATCAATGAACCCGA	50.0	45.5	58.1	57.9	533-652 bp	1.0	67.0
trnL-CAA-ndhB_p5	GTCTCGCGTGTCTACCATTT	ACCAGGAATATCAATGAACCCG	50.0	45.5	58.0	58.2	546-665 bp	1.0	66.9