Hotspot

rpl20-rps12

LSC · 71306-72095 · 790 bp

Back to Cucumis

Pi 0.0069
MarkerSeek score 45.70
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
790
variable_sites
16
pi
0.0069
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CTTGAATGAGCCCCTCGAAA GAAATGTCACCAAATCGCCC 50.0 50.0 57.9 57.7 946-947 bp 1.0 78.3
rpl20-rps12_p2 TTGAATGAGCCCCTCGAAAG GAAATGTCACCAAATCGCCC 50.0 50.0 57.9 57.7 945-946 bp 1.0 78.3
rpl20-rps12_p3 TTTGTTCTACGTCTCCGAGC GAAATGTCACCAAATCGCCC 50.0 50.0 57.7 57.7 904-905 bp 1.0 76.5
rpl20-rps12_p4 TGTTCTACGTCTCCGAGCTA GAAATGTCACCAAATCGCCC 50.0 50.0 57.6 57.7 902-903 bp 1.0 76.2
rpl20-rps12_p5 TTGTTCTACGTCTCCGAGCT GAAATGTCACCAAATCGCCC 50.0 50.0 58.5 57.7 903-904 bp 1.0 75.5