Hotspot

rps4-trnT-UGU

LSC · 48231-48579 · 349 bp

Back to Cucumis

Pi 0.015

MarkerSeek score 48.70

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 349
variable_sites: 16
pi: 0.015
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TTCCGGTTTTGGGCCTTTTA	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.9	57.9	499-520 bp	1.0	81.7
rps4-trnT-UGU_p2	TTCCGGTTTTGGGCCTTTTA	ATGGTCATCGGTTCGATTCC	45.0	50.0	57.9	57.8	467-488 bp	1.0	81.0
rps4-trnT-UGU_p3	GAAACGAGGCCCTCTGTAAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	438-459 bp	1.0	80.6
rps4-trnT-UGU_p4	TTCCGGTTTTGGGCCTTTTA	CTGTTATGTGAGCCCGCTTA	45.0	50.0	57.9	57.7	520-541 bp	1.0	80.1
rps4-trnT-UGU_p5	TTCCGGTTTTGGGCCTTTTA	TGTTATGTGAGCCCGCTTAG	45.0	50.0	57.9	57.7	519-540 bp	1.0	80.1