Hotspot

psaA-ycf3

LSC · 43916-44628 · 713 bp

Back to Cucumis

Pi 0.00734

MarkerSeek score 47.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 713
variable_sites: 16
pi: 0.00734
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTTCTCGAGAAATGACCCGG	ATCAAGCCGCTGAGTATTGG	55.0	50.0	58.4	58.0	915-927 bp	1.0	78.8
psaA-ycf3_p2	TGTTCTCGAGAAATGACCCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.6	58.0	916-928 bp	1.0	78.7
psaA-ycf3_p3	GTTCTCGAGAAATGACCCGG	AGCCGCTGAGTATTGGAAAC	55.0	50.0	58.4	58.3	911-923 bp	1.0	77.0
psaA-ycf3_p4	GTTCTCGAGAAATGACCCGG	CAAGCCGCTGAGTATTGGAA	55.0	50.0	58.4	58.3	913-925 bp	1.0	77.0
psaA-ycf3_p5	TGTTCTCGAGAAATGACCCG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.6	58.3	912-924 bp	1.0	77.0