Hotspot

trnR-UCU-atpA

LSC · 10530-11064 · 535 bp

Back to Cucumis

Pi 0.015

MarkerSeek score 44.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 535
variable_sites: 26
pi: 0.015
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	CGAAGCTTATCCGGGAGATG	50.0	55.0	58.0	57.9	1556-1580 bp	1.0	84.0
trnR-UCU-atpA_p2	ACTAGAGACGTCGACATCGT	CGAAGCTTATCCGGGAGATG	50.0	55.0	58.0	57.9	1894-1914 bp	1.0	83.6
trnR-UCU-atpA_p3	ACTAGAGACGTCGACATCGT	GATCCCTCCAAACAAGCACA	50.0	50.0	58.0	58.1	1959-1979 bp	1.0	83.3
trnR-UCU-atpA_p4	ACTAGAGACGTCGACATCGT	TGATCCCTCCAAACAAGCAC	50.0	50.0	58.0	58.1	1960-1980 bp	1.0	83.3
trnR-UCU-atpA_p5	ACTAGAGACGTCGACATCGT	CCTGAAGCGGAAGTCCTTTT	50.0	50.0	58.0	58.1	1304-1316 bp	0.181818	46.7