Hotspot

trnS-GCU-trnG-GCC

LSC · 8750-9455 · 706 bp

Back to Cucumis

Pi 0.013
MarkerSeek score 46.80
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
706
variable_sites
27
pi
0.013
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 775-855 bp 1.0 73.8
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 774-854 bp 1.0 73.8
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 775-855 bp 1.0 73.7
trnS-GCU-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 774-854 bp 1.0 73.7
trnS-GCU-trnG-GCC_p5 ACGGAAAGAGAGGGATTCGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.2 58.2 829-909 bp 1.0 72.4