Hotspot

trnL-UAG-rpl32

SSC · 109701-110230 · 530 bp

Back to Ctenium

Pi 0.0287

MarkerSeek score 70.20

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 530
variable_sites: 39
pi: 0.0287
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	TCCTAAGAGCAGCGTGTCTA	ACCAAAAGGTTTTTCTGGGT	50.0	40.0	57.9	55.5	580-594 bp	1.0	67.1
trnL-UAG-rpl32_p2	AGCAGCGTGTCTACCAATTT	ACCAAAAGGTTTTTCTGGGT	45.0	40.0	57.8	55.5	573-587 bp	1.0	66.7
trnL-UAG-rpl32_p3	CCTAAGAGCAGCGTGTCTAC	ACCAAAAGGTTTTTCTGGGT	55.0	40.0	57.8	55.5	579-593 bp	1.0	66.5
trnL-UAG-rpl32_p4	CTAAGAGCAGCGTGTCTACC	ACCAAAAGGTTTTTCTGGGT	55.0	40.0	57.8	55.5	578-592 bp	1.0	66.5
trnL-UAG-rpl32_p5	GCTTGAGCACTGCTTCCTAA	ACCAAAAGGTTTTTCTGGGT	50.0	40.0	58.2	55.5	594-608 bp	1.0	66.4