Hotspot

psaJ-rpl33

LSC · 64238-64611 · 374 bp

Back to Ctenium

Pi 0.031

MarkerSeek score 63.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 374
variable_sites: 29
pi: 0.031
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTGGGGCTTTAGCAGGTTT	TCTAACATCTTTGCCCTTGG	45.0	45.0	57.9	55.3	463-512 bp	1.0	65.2
psaJ-rpl33_p2	TTTGGGGCTTTAGCAGGTTT	TTCTAACATCTTTGCCCTTGG	45.0	42.9	57.9	56.1	464-513 bp	1.0	63.6
psaJ-rpl33_p3	ATGGTTTGGGGCTTTAGCAG	TCTAACATCTTTGCCCTTGG	50.0	45.0	58.4	55.3	467-516 bp	1.0	62.4
psaJ-rpl33_p4	ATGGTTTGGGGCTTTAGCAG	TTCTAACATCTTTGCCCTTGG	50.0	42.9	58.4	56.1	468-517 bp	1.0	60.8
psaJ-rpl33_p5	GTTTGGGGCTTTAGCAGGTT	TCTAACATCTTTGCCCTTGG	50.0	45.0	58.7	55.3	464-513 bp	1.0	60.6