Hotspot

rbcL-psaI

LSC · 55957-56795 · 839 bp

Back to Ctenium

Pi 0.0281

MarkerSeek score 61.80

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 839
variable_sites: 54
pi: 0.0281
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CATTCGACTTCAAGCCGGTA	GACATTGCAATTGCCGGAAA	50.0	45.0	58.0	57.9	832-972 bp	1.0	88.2
rbcL-psaI_p2	CATTCGACTTCAAGCCGGTA	CGCGATTCCGTAGAAAGACA	50.0	50.0	58.0	58.1	1447-1593 bp	1.0	87.9
rbcL-psaI_p3	GCCTGTGTACAAGCTCGTAA	GACATTGCAATTGCCGGAAA	50.0	45.0	57.9	57.9	953-1093 bp	1.0	87.1
rbcL-psaI_p4	GAGCTGTATTTGCGAGGGAA	CGCGATTCCGTAGAAAGACA	50.0	50.0	58.0	58.1	2086-2232 bp	1.0	86.8
rbcL-psaI_p5	GAGCTGTATTTGCGAGGGAA	GACATTGCAATTGCCGGAAA	50.0	45.0	58.0	57.9	1471-1611 bp	1.0	86.7