Hotspot

trnC-GCA-rpoB

LSC · 18488-19512 · 1025 bp

Back to Ctenium

Pi 0.0198

MarkerSeek score 63.30

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1025
variable_sites: 57
pi: 0.0198
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	TTGTGGTCTAATTCGGAGCG	50.0	50.0	58.1	58.0	1471-1500 bp	1.0	84.4
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CCCCACCTACACAAGCAAAT	50.0	50.0	58.1	58.1	1755-1784 bp	1.0	83.3
trnC-GCA-rpoB_p3	TCTGTTGGTAGTTCCCCCTT	TTGTGGTCTAATTCGGAGCG	50.0	50.0	57.9	58.0	1686-1699 bp	0.833333	74.7
trnC-GCA-rpoB_p4	GCCAAAACAAAGCCTCGTTT	TTGTGGTCTAATTCGGAGCG	45.0	50.0	58.1	58.0	1662 bp	0.5	59.6
trnC-GCA-rpoB_p5	GCCAAAACAAAGCCTCGTTT	CCCCACCTACACAAGCAAAT	45.0	50.0	58.1	58.1	1946 bp	0.5	59.0