Hotspot

trnD-GUC-psbM

LSC · 15779-16784 · 1006 bp

Back to Ctenium

Pi 0.0201
MarkerSeek score 59.80
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1006
variable_sites
54
pi
0.0201
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.68
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TCAATTGGTTAGAGCACCGC AGAATGAACAATGCAGTAGC 50.0 40.0 58.6 54.0 726-1116 bp 1.0 52.0
trnD-GUC-psbM_p2 TAGGTATGCCATACACCCCG AGAATGAACAATGCAGTAGC 55.0 40.0 58.7 54.0 759-1149 bp 1.0 51.0
trnD-GUC-psbM_p3 AATTGGTTAGAGCACCGCC AGAATGAACAATGCAGTAGC 52.6 40.0 58.1 54.0 724-1114 bp 1.0 48.6
trnD-GUC-psbM_p4 GTTCAATTGGTTAGAGCACCG AGAATGAACAATGCAGTAGC 47.6 40.0 57.5 54.0 728-1118 bp 1.0 48.6
trnD-GUC-psbM_p5 TCAATTGGTTAGAGCACCGC TAGAATGAACAATGCAGTAGC 50.0 38.1 58.6 54.0 727-1117 bp 1.0 48.6