Hotspot

trnG-UCC-trnE-UUC

LSC · 13611-15181 · 1571 bp

Back to Ctenium

Pi 0.023

MarkerSeek score 68.10

Primer available yes

Label trnG-UCC-trnE-UUC

Feature Metadata

label_name: trnG-UCC-trnE-UUC
feature_type: igs
region: LSC
length_bp: 1571
variable_sites: 95
pi: 0.023
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnE-UUC_p1	ACGAATCACACTTTTACCACT	ACCACTAGACGATAGGGGC	38.1	57.9	55.5	57.9	1522-1639 bp	1.0	51.4
trnG-UCC-trnE-UUC_p2	AGAACGAATCACACTTTTACCAC	ACCACTAGACGATAGGGGC	39.1	57.9	57.3	57.9	1525-1642 bp	1.0	50.4
trnG-UCC-trnE-UUC_p3	GAACGAATCACACTTTTACCACT	ACCACTAGACGATAGGGGC	39.1	57.9	57.3	57.9	1524-1641 bp	1.0	50.4
trnG-UCC-trnE-UUC_p4	AACGAATCACACTTTTACCACT	ACCACTAGACGATAGGGGC	36.4	57.9	56.2	57.9	1523-1640 bp	1.0	49.5
trnG-UCC-trnE-UUC_p5	TACCACTAAACTATACCCGC	ACCACTAGACGATAGGGGC	45.0	57.9	54.0	57.9	1508-1625 bp	1.0	48.8