Hotspot

psbC-trnS-UGA

LSC · 11341-11498 · 158 bp

Back to Ctenium

Pi 0.0253

MarkerSeek score 70.70

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 158
variable_sites: 10
pi: 0.0253
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CATTTGTGGCATGCAGGAAG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	338-350 bp	1.0	85.3
psbC-trnS-UGA_p2	AGCAGGCTTTGAAAAGGGAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	57.9	58.0	303-315 bp	1.0	85.3
psbC-trnS-UGA_p3	CATTTGTGGCATGCAGGAAG	ATAGCCCCGGTCTTGAAAAC	50.0	50.0	58.0	57.9	318-330 bp	1.0	84.9
psbC-trnS-UGA_p4	ATTTGTGGCATGCAGGAAGA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.1	58.0	337-349 bp	1.0	84.8
psbC-trnS-UGA_p5	ATTTGTGGCATGCAGGAAGA	ATAGCCCCGGTCTTGAAAAC	45.0	50.0	58.1	57.9	317-329 bp	1.0	84.3