Hotspot

rps16-trnQ-UUG

LSC · 5601-6695 · 1095 bp

Back to Ctenium

Pi 0.0218

MarkerSeek score 60.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1095
variable_sites: 65
pi: 0.0218
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TTCTGTCTTCTCGAGCTCCA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1324-1486 bp	1.0	85.3
rps16-trnQ-UUG_p2	TTCTGTCTTCTCGAGCTCCA	TGACAGGCATAACATCCACG	50.0	50.0	58.1	58.0	1848-2010 bp	1.0	84.7
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TGACAGGCATAACATCCACG	45.0	50.0	58.1	58.0	1691-1854 bp	1.0	84.6
rps16-trnQ-UUG_p4	TTCTGTCTTCTCGAGCTCCA	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.1	57.9	1897-2059 bp	1.0	84.3
rps16-trnQ-UUG_p5	TCTGTCTTCTCGAGCTCCAT	TGACAGGCATAACATCCACG	50.0	50.0	57.9	58.0	1847-2009 bp	1.0	84.3