Hotspot

ycf1

SSC · 124230-129626 · 5397 bp

Back to Cryptotaenia

Pi 0.00889

MarkerSeek score 42.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5397
variable_sites: 48
pi: 0.00889
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTTTCTGATTGTGCTTGTTCT	ACAAGATTCAAAAGAGCTCT	36.4	35.0	56.7	52.4	221 bp	1.0	42.9
ycf1_p2	TGTTTCTGATTGTGCTTGTTCT	ACAAGATTCAAAAGAGCTCTT	36.4	33.3	56.7	53.3	221 bp	1.0	42.9
ycf1_p3	TTGTTTCTGATTGTGCTTGTTCT	ACAAGATTCAAAAGAGCTCT	34.8	35.0	57.4	52.4	222 bp	1.0	42.9
ycf1_p4	TTGTTTCTGATTGTGCTTGTTCT	ACAAGATTCAAAAGAGCTCTT	34.8	33.3	57.4	53.3	222 bp	1.0	42.9
ycf1_p5	TGTTTCTGATTGTGCTTGTTC	ACAAGATTCAAAAGAGCTCT	38.1	35.0	55.3	52.4	221 bp	1.0	42.9