Hotspot

rps16

LSC · 4831-5924 · 1094 bp

Back to Cryptotaenia

Pi 0.0101

MarkerSeek score 51.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1094
variable_sites: 11
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TTTTGTTTGATTAGGACCAAG	TGTATCGACGGAACCAATGAC	33.3	47.6	52.2	58.1	1198-1202 bp	1.0	40.7
rps16_p2	TTTTGTTTGATTAGGACCAAG	GTATCGACGGAACCAATGACT	33.3	47.6	52.2	57.8	1197-1201 bp	1.0	40.7
rps16_p3	TTTTGTTTGATTAGGACCAAG	TGTATCGACGGAACCAATGA	33.3	45.0	52.2	56.7	1198-1202 bp	1.0	40.7
rps16_p4	ATTTTGTTTGATTAGGACCAAG	TGTATCGACGGAACCAATGAC	31.8	47.6	52.9	58.1	1199-1203 bp	1.0	40.7
rps16_p5	ATTTTGTTTGATTAGGACCAAG	GTATCGACGGAACCAATGACT	31.8	47.6	52.9	57.8	1198-1202 bp	1.0	40.7