Hotspot

rpl16

LSC · 81126-82459 · 1334 bp

Back to Cryptotaenia

Pi 0.0106

MarkerSeek score 55.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1334
variable_sites: 14
pi: 0.0106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AGAAACCAGAACCTGTGATTGT	TGACCTTTTGAAATAATCGCT	40.9	33.3	57.8	53.8	1368-1381 bp	1.0	41.7
rpl16_p2	AGAAACCAGAACCTGTGATTGT	TTGACCTTTTGAAATAATCGCT	40.9	31.8	57.8	54.6	1369-1382 bp	1.0	41.7
rpl16_p3	AGAAACCAGAACCTGTGATTGT	AGATTGACCTTTTGAAATAATCGCT	40.9	32.0	57.8	57.6	1372-1385 bp	1.0	41.7
rpl16_p4	TGCAAAGCAAAGAGTGAAGA	TGACCTTTTGAAATAATCGCT	40.0	33.3	55.8	53.8	1428-1437 bp	1.0	41.6
rpl16_p5	TGCAAAGCAAAGAGTGAAGA	TTGACCTTTTGAAATAATCGCT	40.0	31.8	55.8	54.6	1429-1438 bp	1.0	41.6