Hotspot

psbA

LSC · 220-1281 · 1062 bp

Back to Cryptotaenia

Pi 0.00282

MarkerSeek score 49.60

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.00282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTCGTATATACTAAGACACGGTC	AAATTCGTGTGCTTGGGAGT	41.7	45.0	57.2	58.0	1137 bp	1.0	41.8
psbA_p2	ACTCGTATATACTAAGACACGGT	AAATTCGTGTGCTTGGGAGT	39.1	45.0	56.2	58.0	1137 bp	1.0	41.3
psbA_p3	AACTCGTATATACTAAGACACGGT	AAATTCGTGTGCTTGGGAGT	37.5	45.0	56.8	58.0	1138 bp	1.0	40.2
psbA_p4	AACTCGTATATACTAAGACACGGTC	AAATTCGTGTGCTTGGGAGT	40.0	45.0	57.8	58.0	1138 bp	1.0	40.2
psbA_p5	ACTCGTATATACTAAGACACGGTC	AATTCGTGTGCTTGGGAGTC	41.7	50.0	57.2	58.5	1136 bp	1.0	40.2