Hotspot

petB

LSC · 74846-76241 · 1396 bp

Back to Cryptotaenia

Pi 0.0086

MarkerSeek score 61.40

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1396
variable_sites: 12
pi: 0.0086
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AGCCCATTATCTTTTGGCAGT	CCCCTCAGTGATAAAGGATGG	42.9	52.4	57.9	57.5	1507-1508 bp	1.0	59.8
petB_p2	TCTTTTGGCAGTTCGATCGC	CCCCTCAGTGATAAAGGATGG	50.0	52.4	59.2	57.5	1498-1499 bp	1.0	59.0
petB_p3	CTTTTGGCAGTTCGATCGC	CCCCTCAGTGATAAAGGATGG	52.6	52.4	57.7	57.5	1497-1498 bp	1.0	58.3
petB_p4	ATCTTTTGGCAGTTCGATCG	CCCCTCAGTGATAAAGGATGG	45.0	52.4	56.5	57.5	1499-1500 bp	1.0	56.9
petB_p5	GCCCATTATCTTTTGGCAGT	CCCCTCAGTGATAAAGGATGG	45.0	52.4	56.4	57.5	1506-1507 bp	1.0	55.5