Hotspot

ndhF

SSC · 110466-112697 · 2232 bp

Back to Cryptotaenia

Pi 0.00762

MarkerSeek score 61.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 17
pi: 0.00762
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTCATGAAGGCCTACCGTTG	GGGGGTAGGATCCGGTAAAT	55.0	55.0	58.3	58.3	2359-2365 bp	1.0	75.7
ndhF_p2	AATTGGAATTCCTGTCGGGC	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.5	58.3	2383-2389 bp	1.0	74.3
ndhF_p3	ATGAAGGCCTACCGTTGGAT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.8	58.3	2356-2362 bp	1.0	72.2
ndhF_p4	CTCATGAAGGCCTACCGTTG	GGGTAGGATCCGGTAAATCG	55.0	55.0	58.3	57.3	2357-2363 bp	1.0	71.9
ndhF_p5	CTCATGAAGGCCTACCGTTG	TTGAAAGGGGGTAGGATCCG	55.0	55.0	58.3	58.8	2365-2371 bp	1.0	71.5