Hotspot

rps15-ycf1

SSC · 123765-124229 · 465 bp

Back to Cryptotaenia

Pi 0.0224

MarkerSeek score 67.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 465
variable_sites: 9
pi: 0.0224
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GACCCCTTGTTGTCTTCGTT	TGGAAGCCGTTTTAGTATGT	50.0	40.0	58.0	54.7	516-579 bp	1.0	58.5
rps15-ycf1_p2	GACCCCTTGTTGTCTTCGTT	TGGAAGCCGTTTTAGTATGTT	50.0	38.1	58.0	55.5	516-579 bp	1.0	57.1
rps15-ycf1_p3	GACCCCTTGTTGTCTTCGTT	ACCTATAATGGAAGCCGTTT	50.0	40.0	58.0	54.4	524-587 bp	1.0	56.2
rps15-ycf1_p4	TCAACAGACCCCTTGTTGTC	TGGAAGCCGTTTTAGTATGT	50.0	40.0	57.7	54.7	522-585 bp	1.0	56.0
rps15-ycf1_p5	CAACAGACCCCTTGTTGTCT	TGGAAGCCGTTTTAGTATGT	50.0	40.0	57.7	54.7	521-584 bp	1.0	55.9