Hotspot

trnL-UAG-ccsA

SSC · 114662-114763 · 102 bp

Back to Cryptotaenia

Pi 0.0392

MarkerSeek score 70.80

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 102
variable_sites: 4
pi: 0.0392
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	ACAATGGAAACGATCGAAAAGG	50.0	40.9	58.0	57.6	235 bp	1.0	61.9
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	ACAATGGAAACGATCGAAAAGG	50.0	40.9	58.0	57.6	234 bp	1.0	61.9
trnL-UAG-ccsA_p3	TGAATTCGAACAAGCCGCTA	ACAATGGAAACGATCGAAAAGG	45.0	40.9	57.9	57.6	257 bp	1.0	61.2
trnL-UAG-ccsA_p4	AACAAGCCGCTATGGTGAAA	ACAATGGAAACGATCGAAAAGG	45.0	40.9	58.1	57.6	249 bp	1.0	61.2
trnL-UAG-ccsA_p5	TAGACACGCTGCTCTTAGGA	ACAATGGAAACGATCGAAAAGG	50.0	40.9	57.9	57.6	225 bp	1.0	61.1