Hotspot

rpl16-rps3

LSC · 82460-82613 · 154 bp

Back to Cryptotaenia

Pi 0.039

MarkerSeek score 74.90

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 154
variable_sites: 6
pi: 0.039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAAAACCAACGAGTCGCACA	GGTTCCCCTCCAAACCATTC	45.0	55.0	58.9	58.4	291 bp	1.0	70.8
rpl16-rps3_p2	CCAACGAGTCGCACACTAAG	GGTTCCCCTCCAAACCATTC	55.0	55.0	58.9	58.4	286 bp	1.0	70.6
rpl16-rps3_p3	CGAGTCGCACACTAAGCAT	GGTTCCCCTCCAAACCATTC	52.6	55.0	58.0	58.4	282 bp	1.0	69.9
rpl16-rps3_p4	AAAAACCAACGAGTCGCACA	CCTCCAAACCATTCGAGCTA	45.0	50.0	58.9	57.3	285 bp	1.0	68.9
rpl16-rps3_p5	CCAACGAGTCGCACACTAAG	CCTCCAAACCATTCGAGCTA	55.0	50.0	58.9	57.3	280 bp	1.0	68.7