Hotspot

trnP-UGG-psaJ

LSC · 66620-67006 · 387 bp

Back to Cryptotaenia

Pi 0.016

MarkerSeek score 60.90

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 387
variable_sites: 6
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	CGTTACTGCTCAACGGAGAA	GGTGCCACGGAAAGATATGT	50.0	50.0	57.9	58.0	513-524 bp	1.0	81.8
trnP-UGG-psaJ_p2	AAAACAAACGCGCTACCAAG	GGTGCCACGGAAAGATATGT	45.0	50.0	57.9	58.0	446-457 bp	1.0	81.8
trnP-UGG-psaJ_p3	CGTTACTGCTCAACGGAGAA	TGTCAACGCATCTGGGAAAA	50.0	45.0	57.9	58.0	592-603 bp	1.0	81.6
trnP-UGG-psaJ_p4	AAAACAAACGCGCTACCAAG	TGTCAACGCATCTGGGAAAA	45.0	45.0	57.9	58.0	525-536 bp	1.0	81.4
trnP-UGG-psaJ_p5	CAAAACAAACGCGCTACCAA	TGTCAACGCATCTGGGAAAA	45.0	45.0	58.2	58.0	526-537 bp	1.0	81.0