Hotspot

psbE-petL

LSC · 64707-65825 · 1119 bp

Back to Cryptotaenia

Pi 0.017

MarkerSeek score 62.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1119
variable_sites: 19
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGGTTACCGTTAAAGCTGCT	50.0	45.0	57.9	57.1	1278-1293 bp	1.0	73.9
psbE-petL_p2	ACTGACGAATAACCAACCCG	AGGTTACCGTTAAAGCTGCT	50.0	45.0	57.9	57.1	1291-1306 bp	1.0	73.6
psbE-petL_p3	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1311-1326 bp	1.0	73.2
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGGTTACCGTTAAAGCTGCT	50.0	45.0	57.8	57.1	1201-1216 bp	1.0	73.2
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGGTTACCGTTAAAGCTGCT	45.0	45.0	58.2	57.1	1287-1302 bp	1.0	73.0