Hotspot

trnL-CUA-ccsA

SSC · 113179-113294 · 116 bp

Back to Cryptospora

Pi 0.0517
MarkerSeek score 76.40
Primer available yes
Label trnL-CUA-ccsA

Feature Metadata

label_name
trnL-CUA-ccsA
feature_type
igs
region
SSC
length_bp
116
variable_sites
6
pi
0.0517
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CUA-ccsA_p1 GTTTTGAACAAGTTGCCGCT TCAGTACAATAGAAACGACCGA 45.0 40.9 58.1 57.0 277 bp 1.0 56.8
trnL-CUA-ccsA_p2 AAGTTGCCGCTATGGTGAAA TCAGTACAATAGAAACGACCGA 45.0 40.9 58.1 57.0 268 bp 1.0 56.8
trnL-CUA-ccsA_p3 TAGACACGCTGCTCTTAGGA TCAGTACAATAGAAACGACCGA 50.0 40.9 57.9 57.0 244 bp 1.0 56.7
trnL-CUA-ccsA_p4 GTTTTGAACAAGTTGCCGCT GTCAGTACAATAGAAACGACCG 45.0 45.5 58.1 56.9 278 bp 1.0 56.5
trnL-CUA-ccsA_p5 AGTTGCCGCTATGGTGAAAT TCAGTACAATAGAAACGACCGA 45.0 40.9 57.9 57.0 267 bp 1.0 56.5