Hotspot

rpl32-trnL-CUA

SSC · 112312-113098 · 787 bp

Back to Cryptospora

Pi 0.0306
MarkerSeek score 68.30
Primer available yes
Label rpl32-trnL-CUA

Feature Metadata

label_name
rpl32-trnL-CUA
feature_type
igs
region
SSC
length_bp
787
variable_sites
24
pi
0.0306
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-CUA_p1 TGGACATCGTTGAAAGCTTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 55.9 57.9 909-910 bp 1.0 64.3
rpl32-trnL-CUA_p2 TTGGACATCGTTGAAAGCTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 55.9 57.9 910-911 bp 1.0 64.3
rpl32-trnL-CUA_p3 TGGACATCGTTGAAAGCTTT AGCAGCGTGTCTACCAATTT 40.0 45.0 55.9 57.8 902-903 bp 1.0 63.8
rpl32-trnL-CUA_p4 TTGGACATCGTTGAAAGCTT AGCAGCGTGTCTACCAATTT 40.0 45.0 55.9 57.8 903-904 bp 1.0 63.8
rpl32-trnL-CUA_p5 TGGACATCGTTGAAAGCTTT CTAAGAGCAGCGTGTCTACC 40.0 55.0 55.9 57.8 907-908 bp 1.0 63.7